Talk:Isoelectric point

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Wiki Education Foundation-supported course assignment[edit]

This article is or was the subject of a Wiki Education Foundation-supported course assignment. Further details are available on the course page. Student editor(s): Kj0367. Peer reviewers: Kj0367.

Above undated message substituted from assignment by PrimeBOT (talk) 00:55, 17 January 2022 (UTC)Reply[reply]


Should add an example of pI calculation for lysine. I added a simple method, someone might put a blurb about Henderson-Hasselbach...

Should there be mention of ZPC? Lizz612 21:47, 29 January 2006 (UTC)Reply[reply]

Lead paragraph[edit]

tried to introduce the topic more. Expanded on information and elaborated on some topics. It's not perfect but at least I tried. 19:54, 11 October 2007 (UTC)Reply[reply]

Translation into Chinese Wikipedia[edit]

The 20:00, 12 March 2009 version is translated into Chinese Wikipedia.--Wing (talk) 13:44, 28 March 2009 (UTC)Reply[reply]

derivation of formula needed[edit]

The article pulls the average formula out of nowhere. Horrible, horrible way to introduce the concept. I don't have time to fix it right now, but someone should. John Riemann Soong (talk) 06:49, 13 April 2010 (UTC)Reply[reply]

A full derivation can be found in the pKa article. (talk) 20:05, 20 February 2011 (UTC)Reply[reply]

Equilibrium of neutral and zwitterionic glycine[edit]

From the article: "Glycine may exist as a zwitterion at the isoelectric point, but the equilibrium constant for the isomerization reaction in solution is not known"

   H2NCH2CO2H is in equilibrium with H3N+CH2CO2-"
The reason that one can't find a published equilibrium constant for this reaction is simply because it is a two step process. The equilibrium constants for each step are easily obtained from the published pKa1 = 2.34 and pKa2 = 9.58 for glycine (see table in proteinogenic amino acid):
step 1, protonation of the amino group:
H2NCH2CO2H + H3O+ ↔ H3N+CH2CO2H + H2O
K = 4.0 x 109
step 2, deprotonation of the carboxylic acid:
H3N+CH2CO2H + H2O ↔ H3N+CH2CO2- + H3O+
K = 4.6 x 10-3
There is no particular order for the two steps, and deprotonation of the carboxylic acid can just as easily precede protonation of the amino group.
The overall equilibrium constant for the two step process is the product of the two K values shown.
K = 1.9 107
So there is one molecule of H2NCH2CO2H for every 19 million zwitterion H3N+CH2CO2- (talk) 18:31, 20 February 2011 (UTC)Reply[reply]

Wrong charge states for adenosine monophosphate.[edit]

There is a problem with the pKa for phosphate esters, in that the first deprotonation of the phosphoric acid group is almost a strong acid (pKa < 1) and very difficult to measure. For this reason, phosphate esters are often quoted with a single pKa, but the ionization is for the second deprotonation, typical pKa 6-6.5

HOPO2-OR + H2O ↔ OPO22-OR + H3O+

The quoted pKas for 5'-AMP are 0.9, 3.8, 6.1, and should be attributed as

pKa1 = 0.9 - first phosphoric ester ionization
pKa2 = 3.8 - deprotonation from adenine-H+
pKa3 = 6.1 second deprotonation of hydrogen phosphate ester

That would make the charge states

AMP+ ↔ AMP ↔ AMP- ↔ AMP2-

so pI = ½(0.9 + 3.8) = 2.4

See Data for Biochemical Research by RMC Dawson et al. Oxford. (talk) 05:07, 25 February 2011 (UTC)Reply[reply]

The exact page reference for the above is Data for Biochemical Research by RMC Dawson et al 2nd Ed. Oxford (1969) pp. 103-114. The same information is in the 3rd Ed., but I don't have the page numbers. (talk) 00:31, 21 April 2011 (UTC)Reply[reply]

Although free adenine can be protonated twice, formation of the N-ribofuranoside in adenosine deactivates the stronger of the two basic N atoms leaving only the pKa 3.8. (talk) 18:51, 1 March 2011 (UTC)Reply[reply]

What I find confusing[edit]

In the first paragraphs, a surface is mentioned and a solid, but later, the substance going in and out of solution.

Should I be imagining a solution with various ion species, or a surface equilibrium process -- or is it all happening at once? ? If so, where is the solid in the concentration pictures?

Is the charge on the solid or is it some kind of aggregate charge in the liquid?

I would appreciate it if the main components of the system were laid out and described before the ancillary stuff. (talk) 18:33, 24 September 2014 (UTC)Reply[reply]

Make more clear[edit]

मेक ए स्मॉल क्लियर टू अंडरस्टैंड टू राइट अ (talk) 16:52, 5 January 2023 (UTC)Reply[reply]